大鼠神经肽Y（NP-Y）ELISA试剂盒

检测范围：    96T

5ng/L -120ng/L    

使用目的：

大鼠神经肽Y(NP-Y)ELISA试剂盒 用于测定大鼠血清、血浆及相关液体样本中神经肽Y（NP-Y）含量。

实验原理

大鼠神经肽Y(NP-Y)ELISA试剂盒 应用双抗体夹心法测定标本中大鼠神经肽Y（NP-Y）水平。用纯化的大鼠神经肽Y（NP-Y）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入神经肽Y（NP-Y），再与HRP标记的神经肽Y（NP-Y）抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的神经肽Y（NP-Y）呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中大鼠神经肽Y（NP-Y）浓度。

试剂盒组成

标本要求 

1．标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融

2．不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的（HRP）活性。

Calculate

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.