大鼠活化蛋白C（APC）ELISA试剂盒

检测范围：96T

3.0 IU/L - 120IU/L

使用目的：

大鼠活化蛋白C(APC)ELISA试剂盒 用于测定大鼠血清、血浆及相关液体样本中活化蛋白C（APC）活性。

实验原理

大鼠活化蛋白C(APC)ELISA试剂盒 应用双抗体夹心法测定标本中大鼠活化蛋白C（APC）水平。用纯化的大鼠活化蛋白C（APC）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入活化蛋白C（APC），再与HRP标记的活化蛋白C（APC）抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的活化蛋白C（APC）呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中大鼠活化蛋白C（APC）活性。

试剂盒组成

标本要求 

1．标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融

2．不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的（HRP）活性。

操作步骤

• 标准品的稀释：本试剂盒提供原倍标准品一支，用户可按照下列图表在小试管中进行稀释。

Important notes

• The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
• washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
• add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
• if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
• Closure plate membrane only limits the disposable use, to avoid cross-contamination.
• The substrate evade the light preservation.
• Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
• All samples, washing buffer and each kind of reject should according to infective material process.
• Do not mix reagents with those from other lots.

Storage and validity

1．Storage：  2-8℃.

2．validity： six months