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大鼠白细胞介素8(IL-8/CXCL8)ELISA试剂盒

更新时间:2021-06-27

简要描述:

大鼠白细胞介素8(IL-8/CXCL8)ELISA试剂盒 应用双抗体夹心法测定标本中大鼠白细胞介素8(IL-8/CXCL8)水平。用纯化的大鼠白细胞介素8(IL-8/CXCL8)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入白细胞介素8(IL-8/CXCL8),再与HRP标记的白细胞介素8(IL-8/CXCL8)抗体结合,形成抗体-抗原-酶标抗体复合物,经过*洗涤后加底物TMB显色。

厂商性质:代理商浏览量:91

检测范围:                                                          96T

15ng/L-480ng/L

 

使用目的:

大鼠白细胞介素8(IL-8/CXCL8)ELISA试剂盒 用于测定大鼠血清,血浆及相关液体样本中白细胞介素8(IL-8/CXCL8)含量。

实验原理

大鼠白细胞介素8(IL-8/CXCL8)ELISA试剂盒 应用双抗体夹心法测定标本中大鼠白细胞介素8(IL-8/CXCL8)水平。用纯化的大鼠白细胞介素8(IL-8/CXCL8)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入白细胞介素8(IL-8/CXCL8),再与HRP标记的白细胞介素8(IL-8/CXCL8)抗体结合,形成抗体-抗原-酶标抗体复合物,经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白细胞介素8(IL-8/CXCL8)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中大鼠白细胞介素8(IL-8/CXCL8)浓度。

试剂盒组成

1

30倍浓缩洗涤液

20ml×1瓶

7

终止液

6ml×1瓶

2

酶标试剂

6ml×1瓶

8

标准品(960ng/L)

0.5ml×1瓶

3

酶标包被板

12孔×8条

9

标准品稀释液

1.5ml×1瓶

4

样品稀释液

6ml×1瓶

10

说明书

1份

5

显色剂A液

6ml×1瓶

11

封板膜

2张  

6

显色剂B液

6ml×1/瓶

12

密封袋

1个

标本要求 

1.标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融

2.不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。

 

    Important notes

    • The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
    • washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
    • add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
    • if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
    • Closure plate membrane only limits the disposable use, to avoid cross-contamination.
    • The substrate evade the light preservation.
    • Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
    • All samples, washing buffer and each kind of reject should according to infective material process.
    • Do not mix reagents with those from other lots.

    Storage and validity

    1.Storage:  2-8℃.

    2.validity: six months

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