大鼠可溶性P选择素试剂盒

大鼠可溶性P选择素试剂盒 检测范围： 96T

0.5μg/L -20μg/L

大鼠可溶性P选择素试剂盒 使用目的：

本试剂盒用于测定大鼠血清、血浆及相关液体样本中可溶性P选择素(sP-selectin)含量。

实验原理

本试剂盒应用双抗体夹心法测定标本中大鼠可溶性P选择素(sP-selectin)水平。用纯化的大鼠可溶性P选择素(sP-selectin)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入可溶性P选择素(sP-selectin)，再与HRP标记的可溶性P选择素(sP-selectin)抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的可溶性P选择素(sP-selectin)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中大鼠可溶性P选择素(sP-selectin)浓度。

试剂盒组成

标本要求 

1．标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融

2．不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的（HRP）活性。

Purpose

This kit allows for the determination of sE-Selectin/CD62E concentrations in Rat serum, cell culture supernates and other biological fluids

Principle of the assay

The kit assay Rat sE-Selectin/CD62E level in the sample, use Purified Rat sE-Selectin/CD62E antibody to coat microtiter plate wells, make solid-phase antibody, then add sE-Selectin/CD62E to wells, Combined sE-Selectin/CD62E antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat sE-Selectin/CD62E in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit