产品中心您现在的位置:首页 > 产品展示 > 试剂盒 > 人ELISA试剂盒 > 人Ⅱ型胶原(Col Ⅱ)ELISA试剂盒

人Ⅱ型胶原(Col Ⅱ)ELISA试剂盒

更新时间:2021-06-22

简要描述:

人Ⅱ型胶原(Col Ⅱ)ELISA试剂盒
本试剂盒应用双抗体夹心法测定标本中人Ⅱ型胶原(Col Ⅱ)水平。用纯化的人Ⅱ型胶原(Col Ⅱ)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入Ⅱ型胶原(Col Ⅱ),再与HRP标记的Ⅱ型胶原(Col Ⅱ)抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。

厂商性质:代理商浏览量:11

人Ⅱ型胶原(Col Ⅱ)ELISA试剂盒 

检测范围:  96T

1μg/L-60μg/L

 人Ⅱ型胶原(Col Ⅱ)ELISA试剂盒

使用目的:

本试剂盒用于测定人血清样本中Ⅱ型胶原(Col )含量

实验原理

本试剂盒应用双抗体夹心法测定标本Ⅱ型胶原(Col )水平。用纯化的Ⅱ型胶原(Col )抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入Ⅱ型胶原(Col )再与HRP标记的Ⅱ型胶原(Col )抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后底物TMB显色。TMBHRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Ⅱ型胶原(Col )呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品Ⅱ型胶原(Col )浓度。

试剂盒组成

1

30倍浓缩洗涤液

20ml×1

7

终止液

6ml×1

2

酶标试剂

6ml×1

8

标准品(120μg/L

0.5ml×1

3

标包被

12孔×8

9

标准品稀释液

1.5ml×1

4

样品稀释液

6ml×1

10

说明书

1

5

显色剂A

6ml×1

11

封板膜

2张  

6

显色剂B

6ml×1/

12

密封袋

1

标本要求 

1.标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但避免反复冻融

2.不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。

 

  • Principle of the assay

    The kit assay Human Col  level in the sample, use Purified Human Col  antibody to coat microtiter plate wells, make solid-phase antibody, then add Col  to wells, Combined Col  antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Col  in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Materials provided with the kit

    1

    wash  solution

    20ml×1bottle

    7

    Stop Solution

    6ml×1 bottle

    2

    HRP-Conjugate reagent

    6ml×1 bottle

    8

    Standard120μg/L

    0.5ml×1 bottle

    3

    Microelisa stripplate

    12well×8strips

    9

    Standard diluent

    1.5ml×1bottle

    4

    Sample diluent

    6ml×1 bottle

    10

    Instruction

    1

    5

    Chromogen Solution A

    6ml×1 bottle

    11

    Closure plate membrane

    2

    6

    Chromogen Solution B

    6ml×1 bottle

    12

    Sealed bags

    1

    Specimen requirements

  • extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it cant, specimen can be kept in -20  to preserve, Avoid repeated freeze-thaw cycles.
  • Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

                          留言框

                          • 产品:

                          • 您的单位:

                          • 您的姓名:

                          • 联系电话:

                          • 常用邮箱:

                          • 省份:

                          • 详细地址:

                          • 补充说明:

                          • 验证码:

                            请输入计算结果(填写阿拉伯数字),如:三加四=7
                          在线咨询
                          电话咨询
                          15026946994


                          在线客服